FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_eco_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_eco_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19043775
Sequences flagged as poor quality0
Sequence length25-361
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC245740.129039541792528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATA68550.014474.634295-299
AGGCGCC78952.5931884E-512567.906295-299
GCAATAG40254.043906E-512325.916290-294
ATAGGCG15350.08080.0996290-294
TAGGCGC14751.3847483E-47474.472290-294
CTAATAG72801.3228865E-46814.809295-299
GGCGCCT76551.4626794E-46480.968295-299
AATAGGC44555.448842E-43712.0693290-294
GCGCCTG111305.1531335E-43565.9883295-299
TCAAGGC141504.997498E-43506.135290-294
CAATAGG70901.6126981E-43498.7173290-294
AGCGAAG51656.1033084E-43492.867285-289
AAGCACC164256.7335466E-43020.5059290-294
AAGACGC58309.330948E-42836.5815290-294
GCGAAGA35159.764315E-42822.8625285-289
GTCAAGC68000.00105784262653.0378285-289
GGAAGAC197109.696093E-42517.0884290-294
CTAGAGT56350.00179611122071.5825280-284
TGCGCGG42350.01952.4523275-279
AAGGCTA90050.00222592131836.4542290-294
Produced by FastQC (version 0.11.3)