FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_eco.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_eco.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20668435
Sequences flagged as poor quality0
Sequence length25-357
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG21501.6326667E-650087.715300-304
TATTGGC61650.034935.47300-304
TTGGCGA21905.081911E-632781.918300-304
TGGCGAT27451.596795E-519615.408300-304
GGCGATC23601.967139E-518252.305300-304
ACGTAAC9652.992961E-515942.057290-294
AACGTAA23055.254143E-511679.891290-294
GTACATT79806.7474066E-58996.541295-299
TTAGCGC5051.0592018E-48703.867275-279
CGTAACC15751.3404149E-47597.078290-294
TAGTACG11350.06121.279280-284
TACATTG74302.9244853E-44831.2515295-299
ACTGACG32253.3424844E-44770.259290-294
ATTCGTA155800.04607.9844295-299
ACGGCGC32455.057355E-43904.2358285-289
CGCTGTA24009.4580685E-42894.8545285-289
GTATTGG68000.00107768272639.4263295-299
TTCGTAT136550.02628.795295-299
ATTCAGT103650.00106226682597.4094290-294
TTTAAAC107800.00114902412497.4163290-294
Produced by FastQC (version 0.11.3)