FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_eco_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_eco_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18416935
Sequences flagged as poor quality0
Sequence length25-328
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC28752.2096821E-516674.637295-299
CTCGTGT29502.3264723E-516250.705295-299
CGTCAAG39954.2666226E-511999.8955295-299
AAGACCG43608.469635E-58796.254290-294
CGGACAG98500.06489.2837300-301
TCGTGTG44801.877816E-46114.742295-299
GGACACG99502.6465423E-44818.0483295-299
TGCTCGT28005.343814E-43804.7285290-294
GAAATGT135308.155411E-42834.5647290-294
GTGCTCG49259.832776E-42781.1216290-294
ACCGTCA38700.00102077812752.7751290-294
GACACGG107450.00108009642549.4692295-299
GTGCTGA255408.718384E-42502.719300-301
ACGTGCA97500.02458.44285-289
GGCCCGG157900.00111071922428.8577290-294
CACCCGG101100.00127491222370.8992285-289
TCTCTCG33950.00141705712353.4404280-284
GTCAAGA118950.001323642302.9883295-299
GACCGTC60300.02271.4795290-294
CATTCTA106400.00141207062252.7998285-289
Produced by FastQC (version 0.11.3)