FastQCFastQC Report
Mon 8 Feb 2016
mono6_n5_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22390176
Sequences flagged as poor quality0
Sequence length25-384
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG16700.035159.105330-339
ACGTACG28652.7284841E-1127325.518350-359
TACGTCT27950.021007.41350-359
GTACGTC42250.018529.613350-359
CGTACGT61607.075869E-1012709.028350-359
AGCATCG65506.548362E-1011952.307360-363
GATCGAT34401.8189894E-1211379.014330-339
CATCGAT63151.8189894E-1211157.38360-363
CGATCGA50305.456968E-129338.482330-339
TACGTAC26050.09015.849340-349
CGATGTA29607.2759576E-128816.172330-339
GCATCGA89453.6379788E-128752.109360-363
ATCGATC30951.2732926E-118431.622360-363
CTACGTA31350.08324.042340-349
GTACAGC108309.094947E-127228.773340-349
AGCTACG56903.45608E-115896.6313340-349
ATGTACA137151.7382263E-85708.175340-349
TGTACAG144552.1447704E-85415.954340-349
GCTACGT55005.2750693E-115337.7915340-349
GAGCATC159802.314846E-84899.0996360-363
Produced by FastQC (version 0.11.3)