FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_asp_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_asp_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20344699
Sequences flagged as poor quality0
Sequence length25-363
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG622750.30609939227904037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTAAC104850.020558.342310-314
ACCCTAG126800.016999.543315
TTTAACC84352.508864E-512777.368310-314
ACATAAT57853.540195E-512420.294300-304
TTAACCC63354.245345E-511341.973310-314
TAACCCT65559.090463E-58220.984310-314
AAGTAGA89908.549398E-57992.369300-304
CATAATT74702.9512047E-44809.3306300-304
GAAAACT240102.0327843E-44488.8423305-309
ATTTTTT250852.2188871E-44296.476305-309
AACCCTA101500.04247.3735310-314
ATCCCTC181303.476958E-43963.1223300-304
AGTTTAC70706.344273E-43387.619295-299
AGTAGAA112056.6399033E-43206.2205300-304
ATCGTAC24808.196256E-43104.179285-289
GTAGAAA78807.881118E-43039.3992300-304
GCGGTCT23809.2858385E-42921.5803295-299
AAAACTC213159.611068E-42528.1985305-309
ATAATTT122500.00190443631955.1401300-304
AGCGTAG10850.00213753041947.7201275-279
Produced by FastQC (version 0.11.3)