FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_asp.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_asp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16271274
Sequences flagged as poor quality0
Sequence length25-364
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG460430.2829710814285347No Hit
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC280310.1722729271229776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG39004.5495966E-511620.717300-304
GTCACAT48356.992519E-59373.484300-304
TAGTTGT42351.3411457E-47134.3237295-299
TAGACGG15302.4505335E-45642.178285-289
GTTGCTA55202.2784478E-45473.526295-299
CCGATAC14052.952074E-45161.087290-294
TAGTTTT95202.7108137E-44760.5874300-304
CGGGGCC211852.2374939E-44278.5737305
TTGCTAG43005.069239E-43832.6252295-299
ACCGATA34550.03747.8435290-294
TACTCGG63705.6634453E-43557.3623300-304
CGGTACG5457.626098E-43261.0754275-279
TCACATG74907.829875E-43025.4202300-304
GCGACCA28758.6519244E-43002.6199285-289
GCTAGTT29158.8943227E-42961.4177295-299
ACTCGGG57409.03252E-42871.1304300-304
GGAAGTG164608.103325E-42753.39300-304
GTTTTAG60700.00101008452715.0396300-304
GCGGGCT111959.370717E-42698.8713295-299
CGATACG20400.0010952122692.8577290-294
Produced by FastQC (version 0.11.3)