FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_asp_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_asp_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20798344
Sequences flagged as poor quality0
Sequence length25-365
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG899010.432250759964351No Hit
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC506980.24375979164495018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGT17903.4808745E-639609.92300-304
TTGCTCG37705.146905E-628210.25310-312
GTTGCTC82450.025798.094310-312
CCGTAAT11802.7731647E-516387.156295-299
ATTTACT68355.0751907E-510373.338300-304
TGCTCGC74756.0701146E-59485.186310-312
GTGCAGT137756.8714224E-57720.6997305-309
CCCGTAA42401.3670536E-47166.6196295-299
TTACGGT12101.722971E-46761.1343290-294
GCGTTCG14651.7951913E-46599.605295-299
CGTAATG21551.7656681E-46580.21295-299
GTTTCCG47351.7048667E-46417.417295-299
GGGGAAA171751.0682107E-46192.2935305-309
CGTTACC24752.0184352E-46138.6807290-294
CTTACGG28902.7520309E-45257.1743290-294
TTTACTC76303.1620025E-44646.2485300-304
GTTCGTG77353.249625E-44583.1777300-304
TCGTGCA48153.777601E-44417.555300-304
AATGGGG183053.6399814E-43873.355300-304
TTGAATA80504.9274205E-43774.717295-299
Produced by FastQC (version 0.11.3)