FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22023773
Sequences flagged as poor quality0
Sequence length25-365
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA13907.5450534E-630079.457300-304
CGCGTAG9101.4228661E-522972.773300-304
TGGCCAT314800.07968.954305-306
CGTCGTG87252.9725535E-44792.0283300-304
TACATTA89353.1173602E-44679.4004300-304
TTATCGG12953.75864E-44612.294285-289
GGCCATC324352.7388963E-43867.1602305-306
CCTGATA78358.788346E-42910.7466295-299
ATGTTCG81550.02796.5295295-299
ATTAGAC50109.995906E-42781.7998300-304
ACATTAG79500.0010857612629.588300-304
ATATACG12400.00136187492437.4534280-284
CATTAGA75050.00175917572089.1296300-304
GTCGTGG100250.00172641352085.309300-304
GCCGCGT29150.00249447561792.9008295-299
CTTAACG26050.00252724071783.3416290-294
ACCTAAT62050.00253513731757.7878290-294
GACGGAA132603.2581538E-61719.8867295-299
CTGATAC67000.0026987011701.9177295-299
GCATAAC37250.00281664171683.6421290-294
Produced by FastQC (version 0.11.3)