FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_can_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_can_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12791976
Sequences flagged as poor quality0
Sequence length25-361
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG689860.5392911931667164No Hit
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC352190.27532102937028646No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA327860.256301293873597No Hit
TGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA136660.10683259568341905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAGT27655.9758182E-510472.081300-304
CGATTCG12400.05837.763290-294
GCGATTC22800.05772.5884290-294
CGTCGTT6202.9743282E-45189.123280-284
AACGCCC56059.127689E-85165.977300-304
CGACGCG7053.8457097E-44563.4834280-284
CCGCAAA45803.4429296E-44515.799295-299
AAAAATA363450.03983.396310-311
ACATACA52054.4466442E-43973.5562295-299
GTCATCA121603.467271E-43968.6545305-309
GACGTGT22807.7190687E-43174.9236290-294
TTGCGCC91650.03159.335300-304
AGCTTCG65907.1276753E-43138.446295-299
CGCAAAA34158.29401E-43028.1638295-299
TGCTCTC162056.1575876E-42978.0215305-309
CATACAG39900.0011321792591.7744295-299
CCCAATA56050.02348.1714290-294
CGATTAT8800.00154677342284.983275-279
GAGAGTC63500.0014180192279.9453300-304
TGCCAAT59200.00150634082223.2266290-294
Produced by FastQC (version 0.11.3)