FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_can.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_can.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13603513
Sequences flagged as poor quality0
Sequence length25-327
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCG433670.31879265304484217No Hit
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC326250.23982775625678454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGT23351.1399206E-521888.188305-309
GGGTGTA39001.0600179E-519657.277315-317
TAAACTA47151.5493424E-516259.466315-317
GGTGTAG32552.2151396E-515701.665315-317
TTGTCTA65102.9535613E-511776.249310-314
TTAAGTT60657.690515E-58426.862305-309
GTGTAGG56851.3513715E-46742.6016315-317
ACAAGCG50701.7913118E-46048.393300-304
TAAGTTG51251.8303862E-45983.4834305-309
TGGTCCG28552.044848E-45967.1826305-309
GTTGGTC55552.150407E-45520.3154305-309
ATGTGCC100702.1200457E-45075.3643305-309
GTCTAAA51152.734815E-44995.984310-314
GGTCCGG28803.178943E-44839.86305-309
TTGGTCC45753.0629672E-44787.7207305-309
CCGGGGT56003.2780008E-44563.2964310-314
TCTAAAC43553.7005736E-44400.883310-314
CGGGGTG44803.9160418E-44278.0903310-314
AGTTGTC46005.308128E-43703.545305-309
GTCCGGG227800.03365.3809310-314
Produced by FastQC (version 0.11.3)