FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_can_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_can_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14773802
Sequences flagged as poor quality0
Sequence length25-335
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTTAG39902.9178433E-513680.905305-309
AGTAAAA129150.012679.863310-314
CTTTAGT38409.008325E-58529.189305-309
GTAAAAA103506.544593E-57911.133310-314
TTTAGTA36901.2477278E-47396.587305-309
TTAGTAA39001.9512637E-45998.551305-309
TAAAAAC105352.0341063E-45181.4727310-314
ATGCGCG6503.1616195E-45038.7827285-289
TAGTAAA46203.6508578E-44430.748305-309
ACTCTTG53753.7062156E-44352.437300-304
AAAAACC108054.2792188E-43788.997310-314
CCGGGCG71156.493939E-43288.0322300-304
TAACGTA5909.899649E-42861.4438290-294
TCGCGCT20550.00153469712276.8223290-294
CCATCAA242100.00107416242254.722305-309
GATGTAC43100.00154305392235.027295-299
TGCTTAA46750.00181543242060.5276295-299
TTAACGT12450.00215649811934.3308290-294
CGCGGGC47200.0020818231927.5005300-304
AGGTACG10700.00220957631913.0894280-284
Produced by FastQC (version 0.11.3)