FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_ctr.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_ctr.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23376665
Sequences flagged as poor quality0
Sequence length25-346
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTCG9808.596711E-629103.732290-294
TAGTCGA10604.2743446E-513453.61290-294
TTATCCA108208.7327106E-57908.0376295-299
AATTCGT31402.9415512E-45109.373285-289
TAAGTTG71454.569156E-43991.8345290-294
GTCGAAT20756.0049526E-43638.482290-294
TAGCGTG11106.175105E-43613.3853275-279
CAGAACA299606.6950923E-42855.9736300-304
CGTTACT59050.00104019142716.9233285-289
TATCCAG164250.00100603562604.7173295-299
CGGGCAG693700.02466.9155305-308
GTGAATT117250.00123032852432.5505290-294
CCCGCCG730700.02342.0300-304
TACAATC123200.00135835122315.0696290-294
GGCAGGT143350.00143034852238.3582305-308
CATGACC773450.02212.5532295-299
AGTCGAA47200.02091.7122290-294
TCGAATC32800.00219281861908.7961290-294
GCACTAG33600.00230107131863.3486290-294
TCATGTA90050.00241875531781.6136285-289
Produced by FastQC (version 0.11.3)