FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_eco_atra.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_eco_atra.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14741789
Sequences flagged as poor quality0
Sequence length25-348
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA23705.3993594E-511244.099290-293
GCGTGCT58756.635868E-59071.835290-293
CGTAAAC10802.2423078E-45921.892275-279
ATCGTAC19302.1721283E-45917.5093280-284
GAGCATA49152.3220635E-45421.875290-293
GCATATC29503.6804928E-44516.6978290-293
GGAGCAT129703.234068E-44109.2544290-293
GTGCTAT51706.1658E-43436.3013290-293
CGTACTT12707.65794E-43228.1667280-284
TCTAATA60708.499128E-42926.8285-289
TCGTACT20559.497497E-42881.6992280-284
TCTCCGG41500.00100419672751.9983280-284
TCTATAG28750.00158872442224.5718275-279
ACTTTAC84150.00163333582111.1917285-289
TCTACGT19850.0018705862065.376270-274
CCATGAA258850.0012880782058.9927290-293
TGCTATC68700.00199574191939.4844290-293
ATGGTCG30950.00285353491666.4868285-289
GATCGTA9150.00292843491664.232275-279
GACGGGG113600.00297636751563.88285-289
Produced by FastQC (version 0.11.3)