FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_eco.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_eco.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13152262
Sequences flagged as poor quality0
Sequence length25-347
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGA22105.248481E-627935.93280-284
TGTCGAA20902.8163275E-514769.954280-284
GTCGAAC15653.947793E-513149.82280-284
GAACCTG122100.010112.761285-287
CGAACCT15651.1842979E-47889.892280-284
ACCTGCG55559.947765E-57409.349285-287
AACCTGC101151.0994638E-46103.6484285-287
AGGGATT41051.8107003E-46015.923275-279
CATAGAG41151.8195325E-46001.303275-279
AGCATAG22652.4805934E-45451.5146270-274
ATCGTGG52752.9899023E-44681.585275-279
GCAAGCT57553.5587652E-44291.114275-279
TTTGGAA159852.7458346E-43862.2712280-284
GTCAAGC36355.1109405E-43774.318275-279
TTGGAAG86554.829408E-43566.6323280-284
ATAGGCG9155.4632565E-63460.1882260-264
GCATAGG19957.312259E-43257.5334270-274
CGACGCG6008.045005E-43166.0723255-259
TGTCAAG82057.233502E-43009.7942275-279
CATAGGG16050.0010461482747.5925270-274
Produced by FastQC (version 0.11.3)