FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_eco_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_eco_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14328322
Sequences flagged as poor quality0
Sequence length25-348
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG381470.26623494363122213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATTA39409.8175144E-58170.122305-309
AACCGTC19251.2889122E-47601.0117290-294
AGGCATT82501.2913521E-46503.0874305-309
AGATTTC84801.3643572E-46326.7065305-309
CCGTACG10002.0552779E-46190.4385280-284
GCACGTC27502.6303524E-45320.7075290-294
CGTCATA8503.5637294E-44733.8647290-294
AGCGATA12753.8858218E-44508.443285-289
GCATTAT51703.549639E-44447.4004305-309
CATTATA41503.9208189E-44309.275305-309
TAGGACT34454.1277724E-44247.2993290-294
TCATACC50754.5604288E-43964.3206295-299
CAGATCG47356.379912E-43399.185305-309
CAGCGAT31906.756573E-43363.6658285-289
TGAGCCG62506.916415E-43219.028295-299
GCGATAG13659.6608157E-42875.9297285-289
TGAGCAG189156.78782E-42836.398305-309
CACGAGT22150.00100824382794.7805280-284
CATAGTT39250.00102283882733.782285-289
ACCGTCA28750.00120729842544.6863290-294
Produced by FastQC (version 0.11.3)