FastQCFastQC Report
Mon 8 Feb 2016
mono6_n6_vitd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n6_vitd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13234576
Sequences flagged as poor quality0
Sequence length25-335
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA237840.17971108405739633No Hit
TGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC218900.16540008535218656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACATC86450.018519.283310-312
TCTGTTT107750.014858.394305-309
TACATCT55051.9371188E-514541.253310-312
CTGTTTA86804.8159403E-59222.304305-309
CGACGGA12601.3800265E-47474.285290-294
TGTTTAC84151.3578628E-46341.8184305-309
ATAACGT9102.0006605E-46283.328295-299
GTTTACA64501.5954713E-46205.395305-309
ACATCTC107202.2036013E-44978.209310-312
GCCATCG82452.6070199E-44854.433300-304
TTTACAT73753.4763673E-44341.6733305-309
AGTTGTA48855.490494E-43641.5146295-299
TTTCATC120005.522198E-43335.4300-304
ACGTAGT9458.7888836E-43025.3062280-284
AGGCCTT143457.8911823E-42790.157300-304
CGGTCCC36400.00115155462587.2527290-294
GTTGTAC38950.00131852942417.869295-299
GAAGGAC194550.00145139282057.3015300-304
CCGATAA88750.02004.3717295-299
CTATAAG50804.143969E-51853.8583290-294
Produced by FastQC (version 0.11.3)