FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_asp.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_asp.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18944944
Sequences flagged as poor quality0
Sequence length20-371
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAT19958.860423E-626332.715295-297
GGTCATT84402.6430645E-512448.76290-294
TCATTCG32906.0240392E-510645.14290-294
TTAACGA104550.010049.501295-297
TTCGTAT173150.06068.0063295-297
AACGATC62702.1878356E-45585.727295-297
GTCATTC108602.6254507E-44837.3633290-294
CGGGGGT44850.04685.2856285-289
ATCCTTT232050.04527.797290-294
CGACGCG11805.583924E-43788.948270-274
CGCTCGT21005.742437E-43706.086275-279
TCGTATT163950.03204.2551295-297
TGACAAT143107.5970165E-42936.898285-289
ATTTGTC152458.622134E-42756.7734285-289
GGGGGTC61900.00129343092424.8218285-289
TGCATGA89350.00133268032351.8193285-289
CGTATTG162800.02151.26295-297
GACAATT102650.0017588962047.1024285-289
GAGCGAT80250.02014.235280-284
ACAATTG76250.00196253721968.4784285-289
Produced by FastQC (version 0.11.3)