FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_asp_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_asp_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21002490
Sequences flagged as poor quality0
Sequence length20-363
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC259780.12369009579340355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG26458.029419E-626079.87290-292
CGTAAGT11456.8208916E-510631.582275-279
GATTCTA72656.0575585E-59495.011290-292
TACGTAC19703.977382E-65252.3794285-289
GATCGAT24203.046731E-45030.2324275-279
TAAACGC15503.4738742E-44768.2896270-274
CTACGTA26450.04602.33285-289
AGCTACG47154.6771576E-43990.0464285-289
ATTCTAG143550.03604.0361290-292
TATACTT72607.258049E-43167.1836280-284
GCTACGT49850.02965.235285-289
TTCTAGA158350.02613.7515290-292
CTATGGC91600.00115536442510.2349280-284
TAAGGCT55050.00157635242211.292275-279
TCGATCG10350.00175268942149.9534270-274
CTCTTAA108350.00161647682122.1736280-284
AATGACA165350.00156863672085.9163285-289
GGCCGAG190100.00207331051814.3413285-289
ATGTACA128050.00225762371795.6853280-284
CAGCTAC195300.0021882741766.0333285-289
Produced by FastQC (version 0.11.3)