FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_asp_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_asp_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18034506
Sequences flagged as poor quality0
Sequence length20-363
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTATCTCTTCGAAAGGCTCACTCTCAAGCACGACTAAGAGCCTTCTG196990.10922949594516201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAC16100.0107511.67300-301
ACGTACG16601.5068617E-652136.684300-301
CGATCGA24950.023125.422285-289
CTACGTA18751.9224282E-518463.336295-299
GATGTAC52701.5187219E-516422.559290-294
ACAGCTA117150.014775.396295-299
GATCGAT20103.313805E-514352.718285-289
AGCTACG46653.5700457E-512368.258295-299
GCTACGT35850.012070.696295-299
AGCATCG37404.5892208E-511570.44280-284
GCATTGA80053.504126E-510811.604290-294
TCGATCG8456.676403E-510781.3280-284
TTTATCG25001.2302978E-47693.0566285-289
CGGGCGT31801.5482368E-46804.001275-279
ATCGATC16001.6797349E-46761.476280-284
CGATGTA24750.05379.76285-289
CAGCTAC168451.551669E-45137.8384295-299
GTCCTAC85802.4152058E-45043.525280-284
ATGTACA105903.6792958E-44086.2556290-294
GCATCGA53000.04082.4004280-284
Produced by FastQC (version 0.11.3)