FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20003109
Sequences flagged as poor quality0
Sequence length20-365
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAG277910.13893340280253436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAT18953.860512E-513626.006285-289
GTTAATT109104.5701865E-59467.014290-292
TTAATTC146052.456905E-44714.601290-292
ATCAGGT66054.6895648E-43909.3535285-289
TAATTCC208400.00100039772478.05290-292
AAAAAAT438357.3777925E-42356.225290-292
AACGATC60050.00166101632149.9817285-289
GCTCTCT139750.0020990781847.6766285-289
CTTAACG17750.00236173741847.2635280-284
GAACGCT39550.00243755131801.0397275-279
ATCGGTT23450.00258552331761.793270-274
GTTCAAT63650.0029544341622.7042280-284
CTTGCTA75750.00418419761363.5280-284
TCAGGTT95750.00422236141348.3696285-289
GTCTGGT202350.0044002641276.0702285-289
ATCCTTA82250.00493290041255.7461280-284
TTGCTAG47450.0057543771176.6027280-284
GCTGTGC229650.0056673791124.3754285-289
ACCGATA23200.00652670251112.9863265-269
GGCGTTA242200.01066.114285-289
Produced by FastQC (version 0.11.3)