FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_can.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_can.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18971929
Sequences flagged as poor quality0
Sequence length20-365
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATTCC120350.015726.907280
TTTCGTG36852.794368E-45136.318270-274
ATTTAGG42753.7607423E-44427.4463270-274
GGACGTA11954.3489778E-44280.749265-269
AAGAGTA94150.04020.6758275-279
ATACTGC48604.8603406E-43894.513270-274
GAGTAGT70951.6370905E-112667.7002270-274
TTCGTGC46550.00151585612258.9011270-274
TGGTCCA170700.00133238532217.6135275-279
TCCAACG26150.00165082193.3289260-264
GGTCCAT99650.02110.4233275-279
CTAGGCA49650.00192728812006.3954265-269
ATAGGTT28856.91216E-111988.0608260-264
AAAACTG198450.00180074531907.5164275-279
TTATAAT99350.0020308031905.1166270-274
GTCCATA88400.01784.2507275-279
ATAATAG43900.00264324251724.586270-274
TAGAAAG61200.00261990541718.1675270-274
AAAATCC224250.00229933951688.0563275-279
CGCCTAG12650.0029299111662.4797260-264
Produced by FastQC (version 0.11.3)