FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_can_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_can_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16739774
Sequences flagged as poor quality0
Sequence length20-373
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG808030.48270066250595733No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA454350.27141943493382886No Hit
GAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAG198010.11828714055518313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA19700.086682.45350-359
CGATGTA22950.074407.164340-349
TACGTAC14901.2473774E-657303.5350-359
TCGATCG6954.0708146E-640950.703330-339
ACGTACG16204.4235767E-635136.715350-359
ATCGATC13309.938518E-625678.863330-339
GTACGTC18451.9125306E-518511.05350-359
TACGTCT18352.8377624E-515509.939350-359
GCATCGA37250.015280.935330-339
CGTACGT28701.5734258E-914874.951350-359
GATCGAT15003.5395686E-514230.37330-339
GATGTAC60552.059934E-514101.11340-349
CATCGAT30403.1153955E-514043.128330-339
CGATCGA22756.106437E-510723.042330-339
AGCATCG44006.526295E-59702.525360-363
TGAGCAT92204.7762485E-59260.545360-363
CTGAGCA188950.09037.546360-363
GTACAGC62002.1596176E-45508.531340-349
ATGTACA104351.8353271E-45454.862340-349
CTGTAGT65052.3773141E-45250.252280-289
Produced by FastQC (version 0.11.3)