FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_can_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_can_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17923308
Sequences flagged as poor quality0
Sequence length20-368
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA437670.2441904139570664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT25803.4013094E-513880.677280-284
GATCGAT16008.633309E-59326.079270-274
ATCACGT19201.2431818E-47771.7324270-274
AGGAAGC235950.07588.927285-286
GGAAGCA171501.5029602E-45220.429285-286
CCGTAGT7904.735133E-44121.075270-274
TTAGAGC36354.4556728E-44105.014270-274
TCACGTA19104.7156517E-44076.0464270-274
GGTTCTC102255.341907E-43502.4104280-284
GAGTAAT43806.4690516E-43406.7869270-274
GTACATA59000.03372.1416275-279
AACCGTA16307.615054E-43230.9768270-274
GAACCGT26158.838822E-42977.1506270-274
GGACGAA68400.02908.7185275-279
ATGGGAG125308.021593E-42858.112280-284
AACCAAT70059.025241E-42840.2048275-279
TCGATCG7700.00103077022801.7637265-269
ATGTACC74750.00102768382661.6238275-279
CCAGTCG28500.00124487072513.133275-279
ATCGCAT16800.00130200972478.6921260-264
Produced by FastQC (version 0.11.3)