FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_ctr.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_ctr.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17898783
Sequences flagged as poor quality0
Sequence length20-363
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAGT120050.014559.687295-296
ACATCCC149700.05837.977290-294
CAGAGTG158401.3455564E-45517.331295-296
CTACAAC187701.8893833E-44656.0747285-289
TCGGTGA34703.5510882E-44579.2256275-279
GAGCACG65453.4455518E-44450.9556280-284
ATCCTTA66703.5784114E-44367.542280-284
GCACGAA25754.266459E-44242.452280-284
CTGGTGA251353.3880462E-43477.005285-289
TGGTGAG127305.213837E-43432.6206285-289
GGTGAGG90706.6166365E-43211.853285-289
CACGAAC29307.871189E-43139.7349280-284
TACAACA151407.3747005E-42886.2126285-289
TTGCTAC55208.985662E-42878.6074275-279
TATAAAT101858.343291E-42860.2363280-284
GGCAATT55609.116348E-42857.8982275-279
GGGACAG160858.323992E-42716.6465290-294
GAGGGAC327000.02672.6152290-294
GACATCT118700.00113319732454.2131280-284
AGGGACA239609.2352094E-42431.6782290-294
Produced by FastQC (version 0.11.3)