FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_eco.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_eco.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18194531
Sequences flagged as poor quality0
Sequence length20-340
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG1139690.6263915239145214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACTA100050.019379.074295-296
GATATCG18402.2132761E-517562.285290-294
AAACTAG85353.1748757E-511358.386295-296
CTTATAT57504.322829E-511239.863285-289
ATGATAT95003.9333892E-510204.612290-294
ACTACGC38150.010164.489280-284
GTCATTA64705.473181E-59989.06285-289
CGTTCAC24309.2642294E-58865.462275-279
TGATATC65953.9240585E-67349.797290-294
TTACGTC8751.9852247E-46331.025265-269
TATACAC37602.2179849E-45729.5405275-279
TCATTAC60102.361152E-45376.8066285-289
TGGGCGA76652.560411E-45059.038280-284
TTATATG64451.7626026E-65013.903285-289
TTATGGG50654.0246506E-44253.321275-279
TAGGTGC19404.526562E-44164.2534270-274
TATATGA60754.5031443E-43989.4575285-289
CATTACT102504.5784822E-43783.1733280-284
GTAGGTG41655.89625E-43580.8967270-274
ATTACTT68207.296586E-43158.808280-284
Produced by FastQC (version 0.11.3)