FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_eco_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_eco_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17315713
Sequences flagged as poor quality0
Sequence length20-363
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAAC148800.012525.141285-288
TTGCGTA6651.03452956E-48758.181270-274
GGGCCTA51501.2509483E-47237.8286280-284
TTTGCGT16451.5315325E-47081.0815270-274
AAACGTT24251.5254613E-46986.8447275-279
GAACGTA18451.926561E-46313.4854270-274
GAGAACA168451.3384155E-45532.03285-288
GTTGACC40654.2862337E-44168.044275-279
TGCGTAA10204.857539E-44060.4375270-274
GTTCATG103155.0181E-43613.6516280-284
GAACAAA137105.31903E-43398.5063285-288
AACGTAT18207.747981E-43200.1045270-274
GTAAAGG53007.2860013E-43196.8108275-279
AGAACAA195050.03185.065285-288
ACGTTGG21957.975168E-43144.7578275-279
AACGTTG33509.0499496E-42928.1082275-279
ACTAGAC39809.885298E-82926.7285270-274
CCTAGAT34900.00109132912670.1157280-284
CCTGTCA141159.3960407E-42640.7947280-284
GGCCTAG74450.00117624642503.3455280-284
Produced by FastQC (version 0.11.3)