FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_eco_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_eco_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16314916
Sequences flagged as poor quality0
Sequence length20-327
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA342250.20977735956470753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATT22004.13835E-512843.494280-284
TGAAAGT134450.012609.457300
CCCTAAT74353.151139E-511401.085290-294
GTATCCG14905.6946927E-511378.131275-279
GATGGTG165000.010274.796295-299
TCATACC60006.156291E-59418.563285-289
AGTAAGA43501.6178776E-46495.561280-284
GAAATGA165551.5622984E-45120.33290-294
ATGGTGA221552.798009E-43826.092295-299
TTTGTGA164804.6442234E-43429.0886285-289
TATCCGA27258.80209E-93274.4404275-279
AAATGAT178655.4576085E-43163.2454290-294
TTCAGTC95657.8217057E-42954.0713280-284
ATACCCT66709.128092E-42824.157285-289
GAGTAGG32250.00101360212766.7751270-274
TGATGGT104309.300242E-42709.0786290-294
TGGTGAA211507.6491194E-42671.933295-299
CATACCC106559.7057916E-42651.8716285-289
AATGATG165559.37257E-42560.165290-294
ATCCGAC26150.00126655842493.5156275-279
Produced by FastQC (version 0.11.3)