FastQCFastQC Report
Tue 9 Feb 2016
mono6_n2_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n2_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20227638
Sequences flagged as poor quality0
Sequence length20-326
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA521880.25800343075152915No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA418940.2071126643654588No Hit
AAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAG356580.17628355816927316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTCC193000.011919.556300-304
ACCCTCG97402.9369938E-511809.417290-294
ACTTCTG239150.09619.379295-299
ATCCACT123901.4257173E-46189.062290-294
CCTTCCC392200.05865.5645305-306
GACTCCT206951.325916E-45558.044300-304
GACGTCA53202.4531622E-45405.25285-289
CATCCAC224551.5610301E-45122.41290-294
TTCTGAC160552.3939043E-44776.237295-299
ACTCCTT176752.9013693E-44338.471300-304
CTTTCGT35005.801525E-43651.5466275-279
CTTCCCT330253.3765315E-43482.9287305-306
CTTCTGA330503.3816457E-43480.294295-299
AGCGATC22008.9136604E-42987.629270-274
TCTGACT205807.8663876E-42794.551295-299
GTTGACG23350.00106316212736.7053280-284
GGTAGCA73750.02599.4062280-284
CGTTTCG6600.00123766872562.9172265-269
ACGTCAT73700.00131133212401.0796285-289
AATAAAC135850.00159941022116.7412285-289
Produced by FastQC (version 0.11.3)