FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_asp.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_asp.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16962120
Sequences flagged as poor quality0
Sequence length20-362
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAACGAGGTGGC262410.15470353941606355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTGA151000.012290.321295
TACGGGA33805.4344695E-510981.293290-294
CTACGGG61605.415176E-510042.417290-294
GTACTCA42058.411081E-58826.818285-289
CCGCCTA26701.5259533E-46950.707285-289
AGTATAT46451.5394771E-46658.9116280-284
CAGAGTC105251.5808424E-45877.5566290-294
GACTCGA25152.737851E-45270.7715275-279
ACGGGAG50552.5524764E-45244.704290-294
ACCGCCT84403.3883238E-44397.7217285-289
CAATGAC87355.4437993E-43541.001280-284
CCCTAGC89105.6640955E-43471.453280-284
TAAAGGA181402.0008883E-113410.214290-294
TCCAAGT98706.950299E-43133.8037280-284
GTATATG55709.738834E-42776.5388280-284
CGAAGTA20350.00124079762533.2224265-269
ATGAAGT156050.0011582282378.5178285-289
AAACTTA160300.02315.4568285-289
CATCTCG58750.00149378022256.3386275-279
TGACAGC147750.00155737622093.4446280-284
Produced by FastQC (version 0.11.3)