FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_asp_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_asp_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15927438
Sequences flagged as poor quality0
Sequence length20-369
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGG68150.025336.275320-329
TTTTGGG120950.014275.876340-349
CCCCGGG91404.5908702E-59445.663320-329
CGTTAGT6651.5309063E-47212.4775280-289
AAAAATT242850.07110.015330-339
TTTTTTT269400.06409.3066350-355
TTTGGGG145751.16740026E-45923.3867340-349
GGGGGTT60802.0313244E-45679.826340-349
AAAATTT158451.3797078E-45448.6187330-339
AAATTTT120252.3838223E-44786.3267330-339
TTGGGGG124852.5696857E-44609.978340-349
CGGGGGA86454.106664E-43994.603320-329
TTTTTCC115454.3944354E-43738.9934310-319
GCGTTAG14355.9740077E-43646.2195280-289
TTTTTTC158954.1650317E-43620.9858310-319
AATTTTT119654.7199696E-43607.7458330-339
TGGGGGT129205.5034575E-43341.0742340-349
TCCCCCG86156.117116E-43340.428290-299
TTTTTTA138556.328788E-43115.603350-355
ACTATGG57158.0755283E-43021.29280-289
Produced by FastQC (version 0.11.3)