FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_asp_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_asp_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15107187
Sequences flagged as poor quality0
Sequence length20-365
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG5652.6959573E-516910.889275-279
TCGTACT18656.002665E-1114515.559280-284
ACCAACC136500.011899.567290-294
CCAAGGG156200.010398.789295-298
CTAAAAT81354.1096744E-59983.35285-289
AGGGGGG45957.866802E-58837.273295-298
CAAGGGG101956.4545624E-57966.115295-298
GGCCAAC115458.277138E-57034.6074285-289
AAGGGGG87900.06159.6167295-298
CCAACCA135551.141015E-45991.4824290-294
CGTACTA26202.082692E-65166.3193280-284
GCTTACG11453.5410776E-44728.649270-274
GCCAACC95053.3659986E-44272.201285-289
CAACCAA128303.0665073E-44220.0337290-294
TAAAATA101403.830746E-44004.6619285-289
TTATACG8655.923238E-43681.947265-269
GTACTAA27456.3627167E-43480.7473280-284
AGTCAAG82856.3930714E-43267.5334280-284
AACCAAG136056.8959186E-42984.7317290-294
AATGCCG32458.891476E-42944.423275-279
Produced by FastQC (version 0.11.3)