FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17321588
Sequences flagged as poor quality0
Sequence length20-355
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCTC97400.013426.813280-281
CACCTCG49052.304805E-513331.005280-281
CGATGAA32259.963092E-58110.2114275-279
TATGAGT43401.804292E-46026.5967275-279
TTGCATC55508.2608813E-42945.4316270-274
TAGCGAT4550.00110341622711.5312240-244
CTGCTTA62300.00104089642623.9397270-274
TGCATCG36400.00152274552245.487270-274
ACGTTTT34050.00169887392133.744265-269
ACCTGAG128100.00157169882041.7979275-279
CGGGCAC51350.00196972931959.0616275-279
GGCGCGT11100.0023043821870.1152245-249
AGGACGC39250.00225729261851.0568265-269
ATGAGTT72250.00224965761810.0646275-279
TCCGTTC20550.00254780171767.7367255-259
AATGCAC95050.01719.8469270-274
GAGTCCA153650.0022610971702.2734275-279
CCGTTAG5250.00306905181628.1005235-239
GTCGTTA7600.00307803461623.3511240-244
GCGCGTT6550.00323526261584.6013245-249
Produced by FastQC (version 0.11.3)