FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_can.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_can.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16090854
Sequences flagged as poor quality0
Sequence length20-320
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA477920.2970134462720251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAC121700.013103.872290-294
TTACTTG42103.4254474E-512626.613285-289
CTACTTA33604.363722E-511865.634290-294
ATTCGCA16955.1822513E-511760.629270-274
CTTAAAT73153.4472134E-510900.487295-299
GACTACT92200.08648.2705290-294
AATAATG190200.08384.549300
TTAAATA66908.649696E-57945.895295-299
TACTTAA46051.3660724E-46926.1294290-294
ACTTAAA235300.06777.4805295-299
ATATGTC82201.3058387E-46466.9146285-289
TACTTAT35251.6809124E-46462.983275-279
TACTTGA44401.9048662E-45986.266285-289
TTCGCAA16752.1687415E-45950.527270-274
TAAATAA71801.9925699E-45552.72295-299
ATATGAT62102.4842133E-45136.0425280-284
TATGTCT55702.9977862E-44771.817285-289
TATGATA42604.2083036E-44159.471280-284
ACTACTT129853.258525E-44093.804290-294
TCGCAAA16354.7524637E-44064.0703270-274
Produced by FastQC (version 0.11.3)