FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_can_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_can_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14183977
Sequences flagged as poor quality0
Sequence length20-323
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATACC38353.177298E-513110.422280-282
AACTATT67909.9600395E-57404.7827280-282
CATACCA38251.5802949E-46572.3486280-282
ATGTATC47452.4318713E-45298.0474275-279
CATGTAA60103.9012823E-44182.901275-279
ACTATTT76006.2384026E-43307.7942280-282
AGTCTCA76906.387019E-43269.0813275-279
GTATCAT32958.207884E-43051.8042275-279
TGTCGCG5600.00107313412748.4587250-254
TGTATCA53300.00112496322572.666275-279
AGACGAG107700.0012527182334.1907275-279
ATGTAAC59250.00139011372314.314275-279
AGAAGAC225700.00110040952227.6682280-282
CTATTTA98400.00146395722189.8289280-282
ACATCGC35500.00190528462023.2784265-269
CATGACT133800.00193336581878.8668275-279
TGATATA39500.00235875461818.3895265-269
GTAACTA43850.01810.4231275-279
ATCATAC38200.00242664011794.8049275-279
CCTTTCC140450.00213029721789.9064275-279
Produced by FastQC (version 0.11.3)