FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_can_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_can_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13975880
Sequences flagged as poor quality0
Sequence length20-315
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG447570.32024459282707063No Hit
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA344330.24637446801203217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCT30106.786866E-624566.615290-294
TCGGCTG37153.1014722E-513269.721290-294
CTGTGTA118550.012474.991295
CGGCTGT37206.219548E-59938.913290-294
ACACATC98907.327044E-57476.7964290-294
AGTTTCG13352.2827087E-45830.5156265-269
TGATTCA51702.0021056E-45721.123275-279
CTATAAG51902.0176252E-45699.0767275-279
CTGTCAT130851.2825777E-45651.167285-289
GATTCAC35603.4173543E-44615.8247275-279
CAAAACA114002.9204172E-44324.2993280-284
AACCGAG68500.04317.9863275-279
GTCATCG68955.34124E-43574.8376285-289
TCCACAC86005.539654E-43439.3264275-279
GTCGTAT4808.8882516E-43020.6501250-254
TAAATTC44858.285998E-42997.69270-274
GGGGTAG28000.00100404982779.9067265-269
GTTGCCA108908.8823534E-42716.0886275-279
TACTCTT50550.0010525732659.6716270-274
CGTGCAC54650.00123022052460.1353270-274
Produced by FastQC (version 0.11.3)