FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_ctr.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_ctr.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20004690
Sequences flagged as poor quality0
Sequence length20-364
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAATT45550.046646.727295-296
CCAATTG78500.013533.493295-296
GAGGATC274500.07740.468290-294
ATTAACG17151.6329886E-46882.9233280-284
TTAACGA159800.06648.1797285-289
TGCAAGT77802.196485E-45462.1035280-284
TCCATTA49753.2332705E-44745.4126280-284
ATCCAAT90402.965519E-44700.793290-294
CTTATTT91403.031488E-44649.362280-284
TAACGAG160450.04414.165285-289
GATCCAA126503.4841648E-44199.1274290-294
AGGATCC263900.04025.6885290-294
TCGTCGA15858.623027E-43046.6855275-279
GGTCTCC150108.175247E-42831.1238280-284
GGATCCA272950.02594.8079290-294
GCAAGTT104050.00141412642268.9502280-284
GGAGCGG189150.00129818382246.6384280-284
TGTTCGT72300.00172584032099.1487275-279
CCATTAA84850.00172398242086.779280-284
GTTCGTC41400.01973.9486275-279
Produced by FastQC (version 0.11.3)