FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_eco.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_eco.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19381897
Sequences flagged as poor quality0
Sequence length20-370
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCCG34751.3150657E-520378.549295-299
CCGCAGG118350.017950.686300-304
GGCAAAT120600.017615.783305-309
AAGCGAG41251.8530403E-517167.383290-294
CGCAGGC70600.015045.776300-304
TTCCCGC44404.293648E-511962.069295-299
TCCCGCA36704.8892078E-511577.458295-299
ATTGGAG215050.09878.929310
AGCGAGT39908.668354E-58874.117290-294
GCGAGTT28301.04657345E-48341.043290-294
GGTGTTG130156.149073E-58161.5967295-299
CAAATTG102451.1430167E-46912.197305-309
GATATTC57650.06141.8433285-289
TCGTTTA11552.5566452E-45573.8257270-274
CCCGCAG72152.834301E-44907.5156295-299
AAATTGG108952.585219E-44874.8594305-309
AGTTCCC238602.0666275E-44451.9355295-299
CAGGCAA123753.3352568E-44291.8457300-304
CGCAGCG51705.335827E-43735.6492280-284
AGCGCGT16706.377016E-43533.705280-284
Produced by FastQC (version 0.11.3)