FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_eco_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_eco_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18157074
Sequences flagged as poor quality0
Sequence length20-364
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCTACACTGTTGGCTGTGAGGAATGCACAGTGTTTCCCTGTTTATC558660.3076817333013017No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA278260.1532515646518817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA10854.3428936E-638846.164285-289
CTTAGTT127200.016567.645295-297
CCGTTCT106954.2197404E-59852.288290-294
TTAGTTG109254.403186E-59644.871295-297
GCGCGAC32450.07215.9023285-289
CGTTCTT101652.2869794E-45182.9917290-294
TAGTTGG164451.8655421E-44271.621295-297
CAGTGAT256852.4337911E-44102.403290-294
AGTGATA142004.462858E-43710.2192290-294
GAAGGTC116505.0064287E-43617.8616285-289
GTTCTTA139757.2039425E-43015.9631290-294
GGTGTTA59858.719954E-42934.2864275-279
AAAAGCC155758.947796E-42706.1372285-289
CTTTACT68700.0011489132556.2888275-279
AAGGTCT94650.00118953422473.9148285-289
TCTTAGT123100.00117374852445.6357290-294
TGCTCAA130150.00131202592313.16280-284
TTTACTA43350.00160101432209.7144275-279
GGTCTGC73900.00211480521901.1317285-289
TTGGGAC108400.00286007721620.0834275-279
Produced by FastQC (version 0.11.3)