FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_eco_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_eco_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19745183
Sequences flagged as poor quality0
Sequence length20-357
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC247330.12526093072928218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAGG60054.5133056E-511000.124285-289
TTATCCT101004.2559743E-59810.259290-294
TATCCTT58708.625148E-58439.831290-294
AAGCAAG274600.07216.578300-303
CTTCCAA148959.256296E-56652.1396295-299
AGCAGCG160951.0807821E-46156.1743295-299
CGTACGG18550.05622.563270-274
GCTAACA89051.9849486E-45563.37280-284
TACGGAG20252.9251765E-45150.546280-284
TACATTT145452.6477905E-44541.4746285-289
AGCAAGA254852.7097267E-43887.9192300-303
GGCCTAC131454.325041E-43768.871280-284
AGCTCAG264802.9254463E-43741.8286290-294
CTACGGA33355.5675086E-43713.779280-284
GTTAGTA28255.69271E-43691.9841270-274
CTAACAC69005.560987E-43589.9863280-284
CCTACGG47356.1726396E-43487.6318280-284
ACATTTA96205.7908375E-43433.251285-289
CGGAGGC98206.0341076E-43363.3274285-289
GCAAGAC198404.926424E-43329.423300-303
Produced by FastQC (version 0.11.3)