FastQCFastQC Report
Tue 9 Feb 2016
mono6_n3_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n3_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17177402
Sequences flagged as poor quality0
Sequence length20-360
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA13000.0143615.53290-294
TCGGTAC14851.0365329E-662862.016290-294
AGGGTAA39150.047688.426300-304
AACCCGG92550.020172.9305-307
CGGTACC40452.3071847E-515385.267290-294
TACCAAG130100.014350.515295-299
CCCGGCG55054.2732445E-511304.887305-307
CCGTCGG23307.1446106E-510016.105285-289
GGGTAAC100254.7238835E-59311.73300-304
CGTCGGT20308.71585E-59197.055285-289
ACAATCA106305.3112522E-58781.759285-289
GCACCGT108400.08611.633285-289
ACCCGGC113056.0071918E-58257.417305-307
AAGGGTA48351.098755E-47722.862295-299
GGTACCA67000.06966.426290-294
GGTAACC105001.5545831E-45926.99300-304
CACCGTC81151.8570924E-45751.7007285-289
ACCGACA46502.1341232E-45735.7974280-284
CAAGGGT90552.312227E-45154.616295-299
CGCTCTA27253.1758065E-44893.8457275-279
Produced by FastQC (version 0.11.3)