FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_asp.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_asp.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18365497
Sequences flagged as poor quality0
Sequence length20-350
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA350210.1906890948826487No Hit
GGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCTCTCCGCTTGCA258700.1408619652384033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACTAG62700.034497.44300-302
CACTAGT34654.2045303E-631211.973300-302
ATCCCTC148200.014595.071295-299
ACTAGTC60600.011897.633300-302
CGACGCG9801.563789E-47119.7817285-289
GGTAATA27351.7287534E-46590.4624285-289
CTCACTA77102.0815598E-45610.868295-299
GCAAATA84502.5002912E-45119.502290-294
TAGCCCG15203.0579977E-45082.212280-284
GTCTATT39503.6057562E-44563.269285-289
ACGCGAT11950.04113.712285-289
TACGTAA13854.721709E-44109.803285-289
ACTTAGC44554.5865995E-44045.9958285-289
TCCCTCA287602.8966018E-43760.4133295-299
TTATTAT116354.740205E-43718.0742290-294
CAAATAG60405.7482126E-43581.1086290-294
CACAGTC126905.6387566E-43408.9673290-294
TATTATC70007.720487E-43089.985290-294
TCGAGAG58751.4040474E-43068.0706285-289
CCCTCAC237455.9230434E-43036.414295-299
Produced by FastQC (version 0.11.3)