FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_asp_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_asp_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21206885
Sequences flagged as poor quality0
Sequence length20-325
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATAAT48908.258932E-622269.887290-294
ATAAACA113950.019113.602295
CATAATA67654.741918E-510731.683290-294
TTTGTTA107654.002527E-510116.094290-294
ATAATAA108902.4574474E-44999.9883290-294
TAATAAA109404.1333024E-43981.7092290-294
ATTAAAC91304.3180774E-43975.8948285-289
AACCCTA92154.3988496E-43939.2202285-289
GCGAAGA43955.203005E-43812.0154280-284
CGGAAAC98850.03672.2222285-289
TTAGTGG46105.724469E-43634.2317280-284
GTATGGG46305.774243E-43618.5332280-284
AATAAAC102205.4105936E-43551.8508290-294
CACCCGG110206.2907446E-43294.0032285-289
GGAAATT137409.779076E-42641.9155285-289
TTACTAT74750.00150492172241.312280-284
TAGTCGG8100.00161751152240.7356260-264
TCGAGAC82300.0018242322035.6998280-284
TGTAGTT54900.0021854031889.1448275-279
TTAAACA105650.00211973471874.1082285-289
Produced by FastQC (version 0.11.3)