FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_asp_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_asp_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20112808
Sequences flagged as poor quality0
Sequence length20-326
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT209070.10394868782121322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACCG66101.4553929E-516776.055295-299
CGTTACT53802.8923854E-513740.98285-289
CTTCCCA274050.08092.6636290-294
ACCGCCG58301.1321179E-47608.2144295-299
TTCCTTA97809.5579235E-57558.945285-289
TCACCGC105501.1122177E-47007.249295-299
CCTTAGT43651.7769275E-46351.0723285-289
TCCTTAG61701.9019836E-45990.8003285-289
CATCACC371650.05967.4277295-299
GCAGTGT128001.6372005E-45775.506285-289
TCCCATC141702.0064108E-45217.1123290-294
GTTACTT71702.56844E-45155.2637285-289
CCGCCGG474050.04678.3975300-304
CGTAGTA13103.8065744E-44575.6025270-274
CTTAGTA51303.94431E-44323.186285-289
CGCAGCG42204.6263266E-44042.6436280-284
TTAGTAG48705.213335E-43794.994285-289
GGGCAGC626400.03540.54305
GACGGCG51506.8898936E-43312.6128280-284
CCCATCA187207.0031185E-42961.798290-294
Produced by FastQC (version 0.11.3)