FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16879615
Sequences flagged as poor quality0
Sequence length20-369
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG600630.35583157554245165No Hit
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC297930.17650284085270904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTA122000.016972.604305-308
GCAAACC100853.8864684E-510266.027290-294
GAGAAGT230600.08979.435300-304
CCAAAGA264650.07824.1357295-299
TTTTTAT155059.186433E-56677.3867305-308
TTTATGC82251.5509699E-46293.7925305-308
TTTTATG132852.023155E-45195.4775305-308
AGAAGTT222801.8968532E-44646.898300-304
CAAAGAG247052.3322391E-44190.766295-299
AAGTTTT140054.4965732E-43696.2827300-304
GAAGTTT218455.4701365E-43159.621300-304
AGTTTTT148108.380142E-42796.2964300-304
CAAACCA199659.137711E-42592.8594290-294
AAAGAGA273108.5492857E-42527.3496295-299
ATGTTGC82400.00142664792284.4854285-289
TCACGAC22800.00162840562215.0808280-284
GATTTAG61950.00175932462089.0413285-289
CACATGT90350.00171517592083.471285-289
TGATTTA96600.0019606441948.6708285-289
TTGTAGT53100.00226188541856.9254280-284
Produced by FastQC (version 0.11.3)