FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_can.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_can.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24171173
Sequences flagged as poor quality0
Sequence length20-370
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG1660010.6867726278737073No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA1037480.4292220323771627No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA322570.1334523566564188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATC21359.7255E-764896.902350-359
TCGATCG12502.0002535E-655421.95350-359
CTACGTA28750.048193.0350-359
TACGTAC22000.031489.748350-359
GTACAGC92250.030039.0360-364
ACGTACG23001.6929871E-520080.418350-359
GATCGAT27803.4626664E-514239.968350-359
GCGTTCG17504.31239E-513195.703300-309
CGGCGAA20808.399594E-59516.132290-299
CGATGTA33651.0871034E-48235.06350-359
CGTCTGA85859.434746E-58069.5913340-349
CGTTCGT22855.3280546E-67579.589300-309
CGATCGA61301.2025492E-47534.2515350-359
TTTAAAC101101.3084248E-46852.3677300-309
GCGTTAC19650.05875.9497300-309
TACGTCT23602.2576132E-45870.9697320-329
ACGTCTG254651.383578E-45440.9927340-349
GTACGTC29602.8598396E-45201.0093330-339
TACAGCT281851.6949323E-44915.9087360-364
AGCTACG118953.018616E-44659.2646360-364
Produced by FastQC (version 0.11.3)