FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_can_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_can_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20647138
Sequences flagged as poor quality0
Sequence length20-371
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG819640.3969751158732024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG27351.1518205E-727756.467360-364
GTACGTC23551.5224941E-921490.145360-364
TACGTCT19302.1937012E-919666.82360-364
CGTACGT47102.8976501E-916117.608360-364
AGCATCG56901.0368949E-613341.64350-359
TCGATCG11408.341885E-913318.234310-319
GATGTAC70852.0015377E-610714.74360-364
ATCGATC18453.5361154E-810286.441350-359
TACGTAC19051.8189894E-127969.967290-299
GATCGAT21205.3647454E-87161.692310-319
GCATCGA58250.06516.218350-359
CTACGTA26450.06377.982350-359
CGATGTA24558.330608E-86184.4346310-319
CATCGAT48606.461578E-75206.717350-359
CGGAATT23402.957411E-45122.398280-289
GCTAACG8804.625133E-44174.1533270-279
ATGTACA107857.057677E-63519.422360-364
CTTCGCA34156.298515E-43509.93280-289
GATCGTA10706.8377296E-43432.9485270-279
CGATCGA46005.47916E-73300.6057310-319
Produced by FastQC (version 0.11.3)