FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_can_vitd.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_can_vitd.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24311219
Sequences flagged as poor quality0
Sequence length20-371
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC641920.2640427039055508No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA641340.2638041309240808No Hit
GAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGA478280.19673221651287826No Hit
GCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAAC244090.10040220525346755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG16450.024953.27360-363
TACGTAC25800.023865.191360-363
CTACGTA33000.013395.659360-363
CGATGTA33450.012271.488350-359
ACGTACG28250.010171.219360-363
TACGTCT23350.07031.7993330-339
ATCGATC23550.06972.0806310-319
GATCGAT27450.05981.512310-319
GTACGTC29900.05491.3887330-339
GATGTAC69500.04724.9644350-359
CGTACGT68951.8189894E-123205.633360-363
AGCATCG65151.8189894E-123150.2786360-363
CGATCGA69050.02972.3481340-349
CATCGAT57350.02862.9907310-319
GCTACGT59100.02778.2153320-329
ATCGATG82200.02496.8447310-319
GAGCATC166101.2732926E-102471.29360-363
GTACAGC86601.8189894E-122369.9844350-359
GCATCGA73500.02233.9116310-319
ATGTACA123551.8189894E-122214.9268350-359
Produced by FastQC (version 0.11.3)