FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_ctr.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_ctr.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15917133
Sequences flagged as poor quality0
Sequence length20-330
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC323620.2033155091435122No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGA274330.17234887715017522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAG20153.6990634E-513584.742280-284
GTATGTA26251.5065838E-46951.938275-279
ACTTAGC26451.9120144E-46209.4336280-284
TTATACG5954.198912E-44381.473265-269
TCATAAC47406.1399804E-43464.9688280-284
TATGTAC29807.334276E-43241.996275-279
ATCATAA86106.753073E-43179.2397280-284
ATATCCA57707.278594E-43162.71275-279
TTGGGTA30707.7839324E-43146.9539275-279
CTCTTTT91407.609985E-42994.8853280-284
AACGTTC13450.00103920382775.2537270-274
CGTTCCC34700.0011187092629.5151270-274
AGCGCTT38700.00139145382357.7307270-274
CGATGAC42100.00164665072167.32270-274
GTACTTA21050.00189143132053.2505275-279
TTACCAT89350.00174520322042.3989275-279
GCGCTTT26600.00213092841929.5057270-274
TAGCTCG6000.0022505331900.9205250-254
CTGTCAT99250.00215330561838.6736275-279
CAGTCGA28251.8754326E-61761.754265-269
Produced by FastQC (version 0.11.3)