FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_eco.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_eco.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20622966
Sequences flagged as poor quality0
Sequence length20-349
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATC47258.076115E-622520.537295-299
ATGCCTA62458.464434E-58519.579290-294
AATGACT131256.2315696E-58107.3936295-299
CTGAAGG305050.06976.531300
CGGTACC30550.06332.959290-294
GCGGTAC42600.06244.6914290-294
GCCGCGT85700.06208.258290-294
TGCGCTC33653.726966E-44517.4927285-289
TTTATAA127703.539135E-44166.3877290-294
TGACTGA133403.8621173E-43988.3633295-299
GTACGGT8205.2165386E-43932.3555265-269
CGCGGTA136600.03894.932290-294
TATGCGG19205.4132333E-43822.1821275-279
GCCTATA55806.1938626E-43467.2383290-294
GACTGAA137506.838299E-43095.5503295-299
ATGACTG230355.7579135E-43079.6482295-299
CCTATAA54858.4872026E-42984.6306290-294
TGCCTAT89408.09392E-42975.6582290-294
TTAAACG24150.00104620372753.8699280-284
GCGCAGG45850.00103400552730.375280-284
Produced by FastQC (version 0.11.3)