FastQCFastQC Report
Tue 9 Feb 2016
mono6_n5_eco_atra.prinseq.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemono6_n5_eco_atra.prinseq.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19008807
Sequences flagged as poor quality0
Sequence length20-361
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCC240890.1267254699361196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATA74757.107885E-58765.438295-299
GTTGGGT63252.5443846E-45179.5776290-294
CGCCGGG395800.04966.27300-304
AGAGTAA73254.7773644E-43833.5437285-289
TGTTTCT188604.5246456E-43474.107295-299
TCAGTAA100856.468318E-43248.4707290-294
TACCGAC22950.03172.1934275-279
TAAATCG11108.0442603E-43162.242265-269
ATTGCGA12450.00101197122819.3481265-269
GCCGGGC382506.2039826E-42569.4766300-304
CCGTAAA28653.3342076E-92541.0762275-279
TGACCCG274059.5531857E-42390.865295-299
GTCTACG20600.00162444682219.0671270-274
CCGGGCA317800.00128466522061.726300-304
ACAAATG159300.00161375152056.5493290-294
GAGTAAA73600.00208965551907.6569285-289
CAGTAAT93750.01906.0845290-294
AGTAATC65050.00215253141888.5948290-294
TGGGTTG70150.00250322351751.2916290-294
CAGGTGT163700.0023856341715.3763285-289
Produced by FastQC (version 0.11.3)